Cat No.
Product name
Quantity
Price
53111
Histone H3 phospho Ser10 ELISA
1×96 wells
¥7,685
Histone Modification ELISAs provide a simple, sensitive method for detecting changes in specific histone modifications from purified core histones or histones isolated by acid extraction. These kits are sandwich ELISAs that utilize a capture antibody against histone H3 and a primary antibody specifc for the modification of interest. A secondary antibody conjugated to horseradish peroxidase (HRP) and developing solutions provide a sensitive colorimetric readout that is easily quantified by spectrophotometry. The assay is performed in a convenient 96-stripwell plate, enabling low or high throughput screening.
Histone H3 phospho Ser10 ELISA (H3S10ph) kits provide everything needed to screen for phosphorylated serine 10 on histone H3 in human and other (wide range predicted) systems. Histone H3 phosphorylation on serine 10 (H3 pSer10) has been correlated with chromosome condensation, making the phosphorylation state of Ser10 an interesting marker for cells undergoing mitosis. The phosphorylation specific histone H3 antibody will not cross-react with phosphorylation of serine 28. To determine histone H3 serine 28 phosphorylation levels, we also offer a Histone H3 phospho Ser28 ELISA. For added convenience, the Histone phosphorylation ELISAs include paclitaxel treated and untreated acid extract for use as positive and negative controls, respectively. pS10 InformationHistone H3 phosphorylation InfoHistone modifications such as phosphorylation, acetylation and methylation at specific amino acid residues on the histone tails that extend beyond the core nucleosome have been found to influence and regulate transcription, chromosome packaging and DNA damage repair. Phosphorylation of serine 10 and serine 28 in the tail of histone H3 occur early in mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. Histone H3 is phosphorylated on serine 10 (pS10) during late S phase or G2 phase, while the phosphorylation of serine 28 (pS28) occurs during prophase. In contrast to serine 10, serine 28 phosphorylation has never been observed in interphase.Histone H3 phospho Ser10 ELISA (H3S10ph)The Histone H3 phospho Ser10 ELISA is capable of detecting the level of histone H3 phosphorylated at serine 10 from core histone preparations or histones purified by acid extraction from tissue or cell samples (Figure 1).